2x YT medium - Sharebiology
The pH of 2x YT medium is maintained at 6.8 ± 0.2 at 25°C; and is adjusted using NaOH and HCl. Weigh the ingredients separately with respect to the volume of the media. Alternatively, commercially available 2x YT agar media formulations can be used.2xYT medium is a nutritionally rich liquid formulation that is used for the propagation of recombinant strains of Escherichia coli. It is the recommended(Here, we are considering 1L of the media).Together, these components provide the necessary growth factors and conditions that allow for rapid growth and replication of bacteriophages (without weakening the host cells). 2x YT medium also facilitates better recovery of and E.scherichia coli following heat shock during transformation.